![The effect of different DNA extraction methods on cccDNA quantification by qPCR and SB in HBV-infected USG mouse liver tissue. (A) Schematic presentation of the experimental design used for the cross-validation. (B) SB analysis on non-digested DNA extracts using HBV DNA probes in three of the labs. (C) qPCR measurements of total HBV DNA and cccDNA in the DNA extracts. Bars depict the median and range across all four labs. (D) qPCR measurements of cccDNA shown separately for every lab, relative to the amount in the +PK DNA extractions. Bars depict the mean of duplicate measurements. +PK, total DNA extraction with proteinase K digestion; −PK, total DNA extraction without proteinase K digestion; cccDNA, covalently closed circular DNA; HBV, hepatitis B virus; Hirt, Hirt DNA extraction, which does not include a proteinase K digestion; qPCR, quantitative PCR; SB, Southern blot. Credit: Gut (2023). DOI: 10.1136/gutjnl-2022-328380 New standards to quantify hepatitis B virus reservoirs in liver cells](https://scx1.b-cdn.net/csz/news/800a/2023/new-standards-to-quant.jpg)
Approximately 300 million people are chronically infected with the hepatitis B virus (HBV), which can cause liver cirrhosis or cancer. Consequently, there is an urgent need for the development of HBV curative therapies. Due to the unique replication strategy of HBV, however, quantification of viral DNA in infected liver cells, which is essential in preclinical and clinical studies, is technically difficult and not standardized.
An international research consortium led by DZIF scientists has now developed recommendations for the optimization, control and validation of quantitative viral DNA measurements, which could be crucial for preclinical and clinical evaluation of therapeutic strategies.
HBV is a DNA virus that specifically infects human hepatocytes. After infection, the DNA genome of the virus is converted by cellular enzymes into covalently closed circular DNA (cccDNA), which forms stable mini chromosomes within the cell nuclei by association with cellular chromosomal proteins. In this form, the viral DNA then serves as a template for the formation of new virus particles.
A major limitation of preclinical and clinical HBV research is the lack of standardized PCR-based methods for the specific quantification of viral DNA present as cccDNA in HBV-infected samples.
As part of an international consortium supported by the International Coalition to Eliminate HBV (ICE-HBV), DZIF scientists at the University Medical Center Hamburg-Eppendorf, Heidelberg University and the Technical University of Munich worked together with the French L’Agence nationale de recherches sur le sida et les hépatites virales (ANRS) and colleagues at Gilead Sciences in the U.S. to compare protocols and develop evidence-based best practices guidance to quantify cccDNA by quantitative PCR.
“When comparing different protocols for quantifying HBV DNA in tissue samples, we found that the coexistence of different forms of viral DNA as well as conditions of sample preservation and treatment strongly affect PCR-based quantification of HBV cccDNA,” says DZIF young investigator Dr. Lena Allweiss, who led the collaboration of the six participating laboratories. Based on the study results, the team developed sample type-tailored methodological recommendations for the optimization, control and validation of the quantitative measurement of HBV cccDNA.
“The study will assist the HBV cure research programs aiming at assessing the impact of therapies on the cellular HBV reservoir in preclinical studies and clinical trials,” says study lead Prof. Maura Dandri, DZIF scientist at the University Medical Center Hamburg-Eppendorf.
More information:
Lena Allweiss et al, Quantification of the hepatitis B virus cccDNA: evidence-based guidelines for monitoring the key obstacle of HBV cure, Gut (2023). DOI: 10.1136/gutjnl-2022-328380
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New standards to quantify hepatitis B virus reservoirs in liver cells (2023, February 6)
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